Influence of Illumination on the Proliferation of Scenedesmus Obliquus

Influence of Illumination on the Proliferation of Scenedesmus Obliquus
In many lakes and lakes in China, nutrients do not become the limiting factor, and light conditions have an important impact on the production and consumption of algae. The illuminance decays exponentially with the depth of water in the water, resulting in a long distance between the incident illumination and the actual illuminance of the algae. In the research, we often discuss the growth of Scenedesmus obliquus by varying the number of algae cells, OD650, pH, and phosphate concentration under different incident illuminations. The light incubator provides different experiments. The required illumination is possible because the illumination in the light box of the incubator can be artificially adjusted. For the specific measurement, we can perform the measurement and recording through a warm illuminance recorder or also called a temperature illuminometer. Because the light incubator will have some deviation during the adjustment process, it is necessary to use the relevant recorder for the actual measurement, but also to improve the measurement accuracy.
The specific experimental results are as follows: In a continuous flow water column test, the specific illumination of S. obliquus at a specific proliferation rate of 0 is between 10000 and 20000 lux, and the specific growth rate at the incident illumination of 20000 lux is only 0.16d- 1, but in the range of 0 to 38000lux of the incident light illumination, the specific proliferation rate increases with the illumination intensity. In the shake flask test, the incident light illuminance of S. obliquus at a specific proliferation rate of 0 was between 200 and 500 lux, that is, the rate of proliferation at an incident light intensity of 500 lux was 0.15 d-1, and was 0 to In the range of incident light intensity of 5000 lux, the specific growth rate becomes larger as the illuminance increases. In the continuous flow column test, when the incident light intensity was 10000 lux, the proliferation of S. obliquus was obviously inhibited, and its inhibition of illuminance was significantly higher than that of shake flask experiments.
Analysis of reasons: (1) The main reason is that in the shake flask experiment, the water depth is about 2cm, there is basically no light attenuation phenomenon; in the water column test, the water depth is about 90cm, due to the light absorption effect of the algae itself and the water body, the light attenuation phenomenon very obvious. (2) In addition, in order to simulate the algae loss rate caused by dilution, protozoa predation, etc. in nature, a continuous flow condition with a daily dilution rate of 10% was set, resulting in a 0.1 d-1 reduction in the rate of increase in specific value.

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